This report is part of a project to characterize cotton gin emissions from the standpoint of stack sampling. The impetus behind this project was the urgent need to collect additional cotton gin emissions data to address current regulatory issues. A key component of this study was focused on EPA emission factors for particulate matter with a particle diameter nominally less than or equal to 10 µm (PM10). The 1996 EPA AP-42 emission factors were assigned quality ratings, from A (Excellent) to E (Poor), to assess the quality of the data being referenced. Emission factor quality ratings for cotton gins were extremely low. Cotton gin data received these low ratings because they were collected almost exclusively from a single geographical region. The objective of this study was to collect additional PM10 emission factor data for 2nd stage seed-cotton cleaning systems at cotton gins located in regions across the cotton belt based on EPA-approved stack sampling methodology, Method 201A. The project plan included sampling seven cotton gins across the cotton belt. Key factors for selecting specific cotton gins included: 1) facility location, 2) production capacity, 3) processing systems and 4) abatement technologies. Five of the seven gins were equipped with 2nd stage seed-cotton cleaning systems. In terms of capacity, the five gins were typical of the industry, averaging 32.6 bales/h during testing. Some test runs were excluded from the test averages because they failed to meet EPA Method 201A test criteria. Also, other test runs, included in the analyses, had cotton lint fibers that collected in the ≤ 10 µm samples. This larger lint material can affect the reported emissions data, but EPA Method 201A does not suggest methods to account for these anomalies. The 2nd stage seed-cotton cleaning system average emission factors for PM10 and total particulate were 0.039 kg/227-kg bale (0.087 lb/500-lb bale) and 0.056 kg/bale (0.123 lb/bale), respectively. System average PM10 and total particulate emission factors were both lower than those currently published in EPA AP-42. The 2nd stage seed-cotton cleaning system PM10 emission rate test averages ranged from 0.41 to 1.80 kg/h (0.90-3.96 lb/h). The ratio of 2nd stage seed-cotton cleaning system PM10 to total particulate was 70.3%.