Media for the Selective Isolation of Several Fungi Associated with the Seedling Disease Complex of Cotton

Donna P. Miller and W.E. Batson, Jr.


 
ABSTRACT

Eight antimicrobial compounds were evaluated for the selective isolation of cotton seedling disease fungi from soil. Potato dextrose agar was used as a basal medium. Three media-. sodium chloride + pentachloronitrobenzene (NaCl-PCNB) (5% + 375 mglL), imazilil (50 mg/L), and thiabendazole + pentachloronitrobenzene (mortect-PCNB) (75 mg/L + 375 mg/L) were selected for soil assay trials. NaCl-PCNB was more effective in isolating Fusarium sp and imazilil medium more efficient in isolating Rhizoctonia solani when compared to standard isolation media for these fungi. Mertect-PCNB selected for Pythium sp but was not as effective as standard media due to inhibitory effects on Pythium growth.



Reprinted from 1988 Proceedings: Beltwide Cotton Production Research Conferences pp. 26 - 28
©National Cotton Council, Memphis TN

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Document last modified Sunday, Dec 6 1998