Genetically engineered plants of commercial cotton varieties were obtained by Agrobacterium-mediated transformation. The approach combined cottons natural susceptibility to Agrobacterium infection with the ability of cotton cells to undergo somatic embryogenesis and plant regeneration. In selected on kanamycin-containing medium, gave rise to transformed calli that were resistant to the antibiotic and expressed the neomycin phosphotransferase enzyme. A second marker enzyme, chloramphenicol acetyl transferase, was also expresses in some tissues. Amplified tissues were plated onto hormone-free medium to promote embryogenesis. Somatic embryos germinated and whole plants also expressed the marker enzymes. We further confirmed our results by Southern blot hybridization.