Various genotypes of Gossypium hirsutum, both commercial and non-commercial were tested over a range of treatments for embryogenic potential. Additionally genotypes of G. barbadense, G. thurberi, and G. arboreum were tested. Hypocotyl tissues from three-day-old seedlings were used as explants. The basal medium was that of Murashige and Skoog supplemented with B vitamins, 100 mg/l m-inositol, 30 g/l glucose, 1.6 9/1 Gelrite and 0.75 g/l MgCl,. The results indicate that G. hirsutum cv. Coker 312 has significantly greater embryogenic response than all other cultivars of G. hirsutum or other species tested. The implications of genotype specificity are discussed.