egregating populations were developed to evaluate the expression of the fuzzless seed alleles, N₁ and n₂. Accession 143 of the Mississippi Obsolete Variety Collection (MOVC) carries the n₂ locus which is recessive to the seed fuzz phenotype. Data from the F₂, BC₁F₁, F₂:F₃, and BC₁F₂ populations of DP 5690 X 143 fit a two loci model for expression of the recessive fuzzless seed phenotype. Expression of the n₂ locus required a second recessive locus which we have designated n₃. The dominant N₃ allele found in DP 5690 reverses the expression of the fuzzless seed phenotype in homozygous n₂ plants. Accession 243 of the MOVC carries the N₁ locus which is dominant to the presence of seed coat fuzz. No variation from expected ratios occurred when the 243 X DP 5690 cross segregated in the F₂, BC₁F₁, F₂:F₃, and BC₁F₂ populations. The N₃ allele had no apparent effect on the expression of the N₁ locus. In a cross between accessions 243 X 143 a wide variation in the lint percent of individual plants was observed, including lines which were completely devoid of lint and fuzz fiber (fiberless). A line was derived from an individual fiberless plant which was designated MD 17 fiberless. In a cross between DP 5690 X MD 17 fiberless, we demonstrated that three alleles, N₁, n₂ and n₃, were involved in the expression of the fiberless phenotype. The involvement of n₂ and n₃ in the expression of the fiberless phenotype was demonstrated in the F₂ progeny of the cross between MD 17 fiberless X 143. This was the first demonstration that N₁, n₂ and n₃ epistatically interacted to produce fiberless seed.