In the course of investigations involving the association of incompatible and nectariless (Rhyne & Tietjen 1999) data suggesting the location of herbicide tolerance on chromosome one was acquired. This paper describes further investigations of the original population and that discussed by Rhyne 2001. The results indicate that herbicide tolerance is apparently associated with normal palisade and white lint. Only one abnormal palisade, brown-linted individual was homozygous herbicide tolerant. Unexpectedly, individuals showing variegation (as named by Kohel, pers comm 2001) were recovered and investigated. The data indicate that sterility is involved with exceptional inheritance when herbicide tolerant genes are present in TM marker stock. Furthermore, the data clearly indicate that in field situations insect-mediated outcrossing is considerable and should not be ignored.