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LOGO: Journal of Cotton Science


Ty3/<em>gypsy</em> Retro-Transposons in Egyptian cotton (<em>G. barbadense</em>)

Authors: Essam A. Zaki and A.A. Abdel Ghany
Pages: 179-185
Molecular Biology and Physiology

The objective of this research was to isolate and characterize Ty3/gypsy sequences in cultivated allotetraploid cotton, Gossypium barbadense L. Using a degenerate oligonucleotide designed to amplify these sequences by PCR, part of the reverse transcriptase gene of Ty3/gypsy retrotransposons was isolated, cloned, and sequenced. These retro-elements exist in the cotton genome in high copy number, and their encoded transcripts were detected. Phylogenetic analysis shows that these retro-elements are heterogeneous and closely related to elements present in other plant species. These results indicate that Ty3/gypsy retro-transposons are standard component of the Gossypium genome, and support the fact that Ty3/gypsy retro-transposons represent a major component of the plant genome.