ABSTRACT
The proinflammatory and prodifferentiation keto elaidic acids which accumulate in airways of cotton workers and are present in airway cells of normal rabbits are readily synthesized from oleic acid and rapidly incorporated into membrane lipids of human promyelocytes and neutrophils. Rates of production and incorporation of these oxidized fatty acids are greatly increased by receptor ligands, e.g., f-met-leu-phe, phorbol diesters, and diglycerides as well as crude extracts of cotton. Also rates of production of these fatty acid mediators in stimulated inflammatory cells parallels rates of production of platelet activating factor. In addition, these keto fatty acids markedly accelerate fatty acid turnover in various lipids, especially in promonocytic cells (LT-937). These observations suggest that the mediator functions of these mono-oxygenated fatty acids may result from alterations in membrane integrity - the result of their esterification to structural lipids in receptor-laden membranes.
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