In a field study, cultivars Deltapines 50 and 90 (G. hirsutum, L.) grown in open-top chambers were exposed to two levels each of soil moisture, CO₂, and O₃. Fiber samples collected at first and second pickings were evaluated for lint length, maturity, and fineness. The two-way interaction of O₃*CO₂ and CO₂*CV showed significant impacts on lint length. Higher CO₂ level appeared to compensate for the reductions in O₃. Plants grown under CF air and treated with 500 µmol CO₂ mol^-1 had higher values than those treated with ambient CO₂ for length at both pickings and for upper quartile length (UQL) by number at second picking. Varietal differences were significant for length under ambient CO₂ and for UQL under both CO₂ levels. DP50 responded to high CO₂ treatment and had higher UQL values than DP90. Fiber maturity [circularity and immature fiber fraction (IFF)], and fineness [cross section area (A), and micronafis] responses to the interactive effects for O₃*CV and CO₂*CV were significant at first picking only. Varietal differences were significant under CF air and 350 µmol CO₂ mol^-1, with DP50 having lower circularity and higher IFF. Also, varietal differences were significant under both O₃ levels for A and under CF air for micronafis. The three-way interaction analysis showed that the M*CO₂*CV interactive effects was significant for maturity and fineness parameters. The results suggest that the availability of carbon improved fiber length and consequently reduced the percentage of short fiber contents for plants grown under well-watered+NF air and limited-water+CF air conditions.