Application of Random Amplified Polymorphic DNA for Identifying Markers of Cotton Fiber Strength Genes

Y.-H. Park, R. J. Kohel, G. R. Lazo


Concentrations of MgCl2 for each of 153 random 10-mer primers were optimized in the range of 1.5 to 4.5 mM in reaction mixtures for polymerase chain reaction (PCR) in generating random amplified polymorphic DNA (RAPD) from cotton genomic DNA. At least 1296 RAPD PCR-derived cotton genomic DNA fragments in the range of 200 bp to 3.5 kb were generated from the 153 primers at the optimized MgCl2 concentrations. The average size of these DNA fragments was 1.3_+0.5kb (mean _+ standard error), and tot total amplified cotton genomic DNA was approximately 1.7 x 106 bp resulting in approximately 0.2% of total cotton genomic DNA. (Continued)

Reprinted from Proceedings of the 1994 Beltwide Cotton Conferences pg. 700
©National Cotton Council, Memphis TN

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Document last modified Sunday, Dec 6 1998