Further studies were conducted in small field trials to further elucidate the potential of a nuclear polyhedrosis virus (AfMNPV) isolated from the celery looper (Anagrapha falcifera) as a control agent for lepidopterous pests of cotton. Insects of interest included Heliocoverpa zea, Heliothis virescens, Spodoptera exigua and Trichoplusia ni. Tests at Shafter, CA were conducted with a fluorescent brightener to determine if field persistence could be increased. M2R reduced the loss of activity of the virus at the two highest applications tested. Time to 50% loss of original activity was extended from 5.5 to 11.5 days. Tests at Maricopa, AZ confirmed that all four species could be infected under field conditions. In addition, the addition of a bait (COAX) increased the percentage of infection of H. zea larvae with all application rates of A@V. At Stoneville, AfMNPV performed as well as two other baculoviruses known to infect H. virescens. However, the addition of either baits or brighteners to the sprays did not provide a clear pattern as to the relative merits of either adjuvant. The interaction of various adjuvants as related to efficacy and persistence of AfMNPV needs further investigation.