Ethephon and Pix growth regulators were used in all combinations to attempt to facilitate once-over spindle pick harvesting. High rates of ethephon used when only 25% of the bolls were open reduced micronaire and slightly reduced yield in two cases. Ethephon applied when 74% of the bolls were open reduced micronaire only for the sixth harvest and resulted in as much increased yield as 14%. There were no significant effects on fiber length or strength. Pix applied alone increased yield 10%. Some interactions occurred for combinations of ethephon and Pix.