Response of cotton fruiting characteristics and yield to the plant growth regulator PIX was examined in Tallahatchie County, Mississippi in 1988. PIX was applied in multiple applications at weekly intervals beginning at pinhead square. Four applications of 2 ounces per acre, two applications of 4 ounces per acre and one application of 4 ounces per acre were applied to respective plots. Plant mapping data and yield data were collected and reviewed to determine the effects of the PIX treatments. Four applications at 2 ounces per acre significantly reduced plant height while both the four applications at 2 ounces and one application at 4 ounces significantly increased yields. While nonsignificant there was a consistent trend for all of the treatments to increase the percent of the total bolls being located in the first position of the fruiting branches, and to increase the percent retention of bolls at the first position.