In 1986 and 1987, Cotton Incorporated organized field studies of the effects of weathering on levels of bacteria and endotoxins that naturally contaminate raw cotton. Samples of cotton fiber from the 1987 microbiological field study were analyzed for organic acids sugars, and sugar alcohols. The samples came from weekly harvests of plots in California, Mississippi, and Texas. The acids and sugars were extracted from fiber with water. The extracts were freeze -dried, derivatized, and analyzed on a capillary gas chromatograph. The effects of time and growing location on levels of these compounds will be discussed. The results will be compared with those from the 1986 microbiological field study.