A wide array of chemical and biological treatments were evaluated for control of Phymatotrichum root rot on cotton. The test site was a Clareville cl soil near Tynan, Texas which has a history of root rot infestation. Ammonium sulfate applied to the soil as a nitrogen source was very effective in reducing the severity of root rot. Data indicated that calcium nitrate accentuated cotton root rot, which increased plant mortality rates and reduced lint yields. Stem drenching with Fe-chelate (Fe-EDDRA) was very effective in reducing the plant mortality rates. Stem drenching with Tilt and LiCl showed promising results in controlling the severity of root rot. Seed coating with Zn + Fe was effective in reducing the severity of root rot disease when combined with ammonical nitrogen. Stem drenching with combinations of two bacteria and a fungus, to some extent controlled the root rot disease. Data indicated that combinations of chemical and biological treatments of soil and plants could be an effective measure in reducing the damage caused by Phatotrichum root rot.