Effects of ginning methods on mechanical properties of cotton fibers were investigated and analyzed. The emphasis is given on the effects of ginning rate and number of lint cleaners on breaking strength and breaking elongation of single cotton fibers. Hand-ginning and machine-ginning were compared with respect to fiber tensile properties. For the preliminary experiment, three groups of cotton fiber samples were taken at the end of three different ginning rates. Both the breaking strength and breaking elongation were higher when the fibers were ginned by hand. Effects of ginning rate on breaking elongation of fiber were significant whereas the effects on breaking strength were not significant. For the main experiment, where the lint- cleaning method was considered as another process condition, the ginning rate was found to be significant for determining the tensile properties for STV 747, whereas the number of lint cleaners played an important role in determining the fiber properties for DPL 33B cotton.