Six cottons were selected from the Southeast to study the influence of varieties and ginning treatments on fiber properties, spinning performance and yarn quality characteristics. Five of these cottons were selected from South Carolina and one from North Carolina. The South Carolina cottons were from the same general area and were produced using typical practices for the area. The North Carolina cotton was a UNR (ultra narrow row) cotton. It was included in the study to determine changes/modifications in ginning that might be necessary in ginning UNR cottons. From this study it appears that varieties had the most influence on fiber properties, spinning performance and yarn quality measurements. The number of lint cleaners was second most important, followed by super mote settings. Super mote settings influenced a number of fiber property measurements. However, super mote settings showed little or no influence on spinning performance and yarn quality measurements.