Potential biological control agents were evaluated for control of seedling diseases of cotton at twelve sites across the cottonbelt. Seedling disease pressure varied across sites and seedling survival ranged from 17 to 86% of seed planted. Several biological agents significantly increased stands of seedlings at one or more sites over the nontreated control. T. virens (TV-117and Tv-111) significantly increased stands at four sites. Rhizoctonia solani was the most prevalent seedling disease pathogen isolated form seedlings.