In open-top chambers, two cultivars Deltapins 50 and 90 (G. hirsutum, L.) were grown in the field conditions and exposed to chronic combinations of 2 levels each of climatic factors soil moisture (-0.05 and -0.1 MPa), CO₂ (ambient and 500 µmol CO₂ mol^-1), and O₃ [carbon-filtered (CF) and non-filtered (NF) air]. The results indicated that plants grown under W-CF-500 µmol CO₂ mol^-1 tended to have greater number of leaves, somewhat elevated LAI, and taller plants. This suggest that availability of C and soil moisture together in combination with other climatic conditions stimulated total biomass accumulation, while CF air inhibited partitioning to fruiting parts. Enriched CO₂ stimulated biomass accumulation and partitioning to fruiting parts under water limited conditions. DP50 exhibited significantly higher total biomass accumulation and fruit parts under most of the factor combinations compared to DP90.