Mainstem node location of bolls had a significant influence on lint weight and all lint quality parameters measured by the HVI. Mean lint weights were relatively consistent until a decline was noted in bolls from higher mainstem nodes. Micronaire values were highest in bolls from the lower mainstem nodes, but declined steadily thereafter. Means for length and strength were low at the first mainstem nodes, increased in the mid nodes, and declined in the upper mainstem nodes. Branch node location also had a significant influence on lint weight and micronaire but not on fiber length and strength. For lint weight and micronaire highest to lowest values were from bolls in the first, second and third fruiting positions, respectively. Branch node location was not significant for length and strength because of a reversal of trends between the branch location and mainstem nodes.