Cultivars, row configuration and harvesting methods were evaluated for 30-inch and 40-inch cotton production systems. Studies were conducted on three location in the Lower Rio Grande Valley of Texas of Texas. Studies concluded that Rio 875 outperformed Stoneville 825 outperformed Stoneville 825 in all row configuration and that 30-inch row configuration increased yields 9% greater than 40-inch cotton. Earliness was increased by use of 30-inch row configuration increased yields 9% greater than 40-inch cotton. In comparing different harvesting methods for 30-inch row configuration (spindle, brush stripper, and brush stripper, and brush stripper with cleaner) both the spindle and stripper with cleaner showed improved bale grade and reduced ginning costs over the stripper without cleaner.