The Effect of Boron Concentration on Cotton Ovule and Fiber Growth In Vitro and Incorporation of UDP [(14)c] Glucose into Fiber Glucans and Lipids

W.M. Dogger and R.L. Palmer


 
ABSTRACT

Inclusion of horon in the medium of in vitro-cultured cotton ovules profoundly affects fiber growth. Fiber yield/ovule was not significantly different from 0.1 to 1.0 mM boron; below 0.1 mM and above 1.0 mM fiber yield was decreased. Deficient or excess of boron in medium resulted in altered types of ovule growth. UDP[(14)C]-glucose (5 µM) is rapidly incorporated into fibers at sufficient and excess levels of boron, with the greatest incorporation occurring, on a dry weight of fiber basis, at 10 mM boron; this was caused by the increased incorporation of UDP[(14)C] glucose into lipids. With [(14)C] glucose (5 µM) as substrate, there was no significant difference in total label incorporated, but the total glucan fraction was reduced 72%, and the cellulose fraction 85% as the boron level in culture increased from 0.1 t 10 mM.



Reprinted from 1986 Proceedings: Beltwide Cotton Production Research Conferences pp. 82 - 84
©National Cotton Council, Memphis TN

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Document last modified Sunday, Dec 6 1998