Equilibrium moisture contents for cotton plant parts (leaves, sticks and burs) were obtained at temperatures ranging from 40 to 176 F in a moving air stream (flow rate of 0.65 ft(3)/m) and relative humidity ranging from 0 to 98%. The target temperatures were 40, 68, 95, 122, 149, and 176øF. Data were obtained for both increasing and decreasing relative humidities for each temperature and plant part tested. The data showed that temperature had only a small effect on the moisture content under increasing relative humidity conditions. However, the data for the decreasing relative humidity portion of the study showed changes in moisture with changes in temperature. This information will be used in conjunction with the desorption and adsorption rate curves to estimate the moisture content of harvested seed cotton during various phases of cotton processing.