The effects of small variations in cotton fiber properties and their machine interactions were studied. Fiber properties of carded and drawn slivers were evaluated, and the slivers were used to produce yarns using both ring and open-end systems. Single-knit fabrics were produced from three yarn types, and the mechanical properties of these fabrics were analyzed. The fabrics were dyed, and their colors were measured. The influence of fiber properties, spinning systems, and yarn characteristics on the color and mechanical properties of resultant fabrics was investigated.