Conservation tillage can conserve natural resources. However, physiographic regions are different in soils, weather, and cropping systems. We investigated a two-year corn-wheat-cotton rotation from 1988 to 1992. The soil was a Norfolk loamy sand (Typic Kardiudult), and the plots were 23 by 61 m arrayed in a randomized complete block design with five replications. Conventional tillage consisted of multiple diskings and cultivations; surface tillage was eliminated for conservation tillage. We subsoil-planted with a Kelley conservation tillage subsoiler and International 900 conservation tillage planters. Planting dates ranged from June 7 to 18, and two years had crop failure because of early freezes. However, the other three years had > 150 days of growth and lint yield ranges of 0.78 to 1.58 bales/ac for conservation tillage and 0.68 to 1.39 bales/ac for conventional tillage. Conservation tillage was significantly greater every year. Delta Pineland 20 had the best lint yields with 1.34 and 1.09 bales/ac for conservation and conventional tillage, respectively. PD 3 had lint yields of 1.06 and 0.98 bales/ac for conservation and conventional tillage, respectively. Selection of early maturing cotton and wheat cultivars will be important for this crop rotation. Further studies are needed to capitalize on the advantages of conservation tillage.