RFLP Analysis of Two F2 Populations

Z.W. Shappley, J.N. Jenkins, C.E. Watson, A.L. Kahler


Two F2 populations of cotton (Gossypium hirsutum L.) were analyzed with restriction fragment length polymorphism (RFLP) technology. An F2 population from the cross of HS46 x MARCABUCAG8US-1-88 was analyzed with 73 probe/enzyme combinations which resulted in 57 polymorphic fragments representing 25 polymorphic loci. Of these polymorphic loci, 14 were codominant and 11 were +/- genotypes. Two linkage groups spanning 123 and 0.5 cM were established with 9 and 2 loci, respectively. The 14 remaining loci were unlinked. Studies are presently being done to associate these molecular markers with fiber traits. In another cross, fifty F2 plants from a population of ST453 x M315 were analyzed with 31 probe/enzyme combinations that resulted in 24 polymorphic fragments. These fragments represented 13 polymorphic loci. Two of these loci were codominant while 11 were +/- genotypes. Six loci were paired into 3 linkage groups of 2 loci each, while the remaining 7 loci were unlinked. These loci are being studied in an attempt to find a molecular marker for root-knot nematode (Meloidogyne incognita (Kofoid and White) (Chitwood)) resistance.

Reprinted from Proceedings of the 1994 Beltwide Cotton Conferences pg. 702
©National Cotton Council, Memphis TN

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Document last modified Sunday, Dec 6 1998