Application of PCR to Identify DNA Sequences of Biological Importance in Cotton Genome

S. Saha, K. M. Soliman, M. Jaggernauth


A genetic linkage map of cotton will expedite the genetic improvement of its quality and productivity. Much effort is currently being directed to develop molecular or biochemical markers. The conventional detection of RFLPs by Southern blot analysis is laborious, costly, time consuming and sometimes difficult with large and complex genome like cotton. Recently, the swift development of methods for a rapid and efficient synthesis of synthetic oligonucleotides with the discovery of polymerase chain reaction (PCR) provided a rapid, safe and efficient way to develop genome mapping program. (Continued)

Reprinted from Proceedings of the 1994 Beltwide Cotton Conferences pg. 701
©National Cotton Council, Memphis TN

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Document last modified Sunday, Dec 6 1998