Transgenic BXN(TM) cotton (Gossypium spp.) strains are resistant to bromoxynil, the active ingredient in Buctril^® herbicide, and will provide growers with a valuable new method of weed control. Testing included 12 replicated yield tests that were conducted across the cotton belt to characterize the yield and agronomic performance of 16 experimental strains and four check varieties. None of the yield test plots was sprayed with Buctril^®. Across all 12 locations, four BXN(TM) strains yielded within the same standard error as the commercial check Stoneville 453. BXN 10215-8 was significantly higher in yield than Stoneville C 315. Data for the 5 Mid-South locations showed that three BXN(TM) strains were not significantly different in yield from the Stoneville variety LA 887, and two strains were not different in yield from the standard mean of adapted checks (mean of Stoneville 453, LA 887, and DPL 5415). The highest yielding variety in the Mid-South tests, Stoneville 453, was only 78 lbs. higher in yield than the highest yielding BXN strain. Yield data from this single year of wide area testing indicates that BXN strains have comparable yield, fiber quality, and Verticillium wilt tolerance to leading commercial checks.