Cotton was heated in a gas-fired pilot plant dryer at temperatures previously shown to reduce endotoxin levels in fiber. Endotoxin levels of dust collected from the heated fiber were measured by the LAL assay. To determine if sufficient dust for animal model evaluation of physiological activity can be recovered from heated fiber, dust content of the heated cotton was determined using a Microdust & Trash Monitor (MTM). The effect of heat treatment on fiber physical properties as measured by High Volume Instrument (HVI) testing was evaluated. Procedures were developed for recovery of dust released during fiber processing in SRRC's pilot plant textile mill.