Cotton (Gossypium hirsutum, L) seed from three Delta and Pineland cultivars (DP-20, DP-50 and DP-5415) were treated with combinations of either carboxin, pentachloronitrobenzene and metalaxyl or triadimenol, metalaxyl and thiram with and without the biological fungicide, Kodiak^® Concentrate containing Bacillus subtilis GB03. These seed were planted in the field and the root growth was monitored over time for three weeks. The application of Kodiak^® Concentrate produced a significant (p=0.1) increase in root area as measured by digital image processing. In addition, the recovery of Fusarium sp. from surface-disinfected roots was reduced and yield was increased in plants derived from Kodiak^®-treated seed.