Transient Expression of b-Glucuronidase Gene in New Hybrid of Fine Extra-Long Staple Egyptian Cotton Seedlings Following Biolistic Particle Bombardment

Osama A. Momtaz and Magdy A. Madkour


 
ABSTRACT

Transient expression of beta-glucuronidase gene in new hybrid of fine extra-long staple Egyptian cotton seedlings (Gossypium barbadense L.) following biolistic particle bombardment has been demonstrated using betaglucuronidase (GUS). GUS expression occupance was derived by double CAMV 35S promoter sequences and bombarded using two types of - accelerated particles (gold and tungsten) with different sizes under two rupture disk pressures (650 psi, and 2000 psi). Different frequency levels of expression were presented in seedling cotyledons. GUS histochemical staining indicated the presence of active enzyme in localized centers (blue spots) when sampled 1hr and 24hrs post-bombardment. GUS expression declined dramatically from 24hrs to 2wks and 4wks postbombardment, but remained detectable in few cotyledons sampled 2wks post-bombardment, however, transgenic plants were not recovered. Direct DNA uptake using the Biolistic PDS-1000/He particle delivery system, provides a useful method to assay transient gene expression in Egyptian cotton seedlings, thus has a potential in the production of transgenic cotton plants.



Reprinted from 1993 Proceedings Beltwide Cotton Conferences pp. 624 - 627
©National Cotton Council, Memphis TN

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Document last modified Sunday, Dec 6 1998