Potential for RFLP Mapping in Cotton

Zachary W. Shappley, J.N. Jenkins, and J.C. McCarty, Jr.


 
ABSTRACT

Sixty-nine bulk-sampled lines of cotton (Gossypium hirsutum L.) were analyzed in 1991 with restriction fragment length polymorphism (RFLP) technology using 75 probe/enzyme combinations. One-hundred-seventy-nine polymorphic fragments resulted from the analysis giving many possibilities for the establishment of molecular markers. In 1992, three crosses (HS46 x MARCABUCAG8US-1-88, HS46 x PD5363, and ST453 x M315) with a high level of polymorphism between the parents were selected for further analysis. Nine individual Fl plants from each cross were analyzed with RFLP'S. The Fl's gave a total of 306 fragments when screened with 73 probe/enzyme combinations. Individual Fl plants within a cross showed polymorphism among themselves with certain probe/enzyme combinations. This variability between Fl's indicates the variability of the parents within a line for those particular DNA fragments. With this large amount of polymorphism that has been detected in cotton, the potential for finding linkages and molecular markers for mapping agronomic and host plant resistant traits is promising. Selfed F2 seed from the Fl plant with the largest number of polymorphisms among each cross will be grown in a 100 plant population. This population will be analyzed with RFLP's and used in F2 linkage studies. These studies will hopefully further genome mapping of agronomic and host plant resistant traits in cotton. With a projection of 50-60 of the 306 fragments to be segregating in any one of the crosses, it is possible to have a molecular marker for several of the 26 cotton chromosomes.



Reprinted from 1993 Proceedings Beltwide Cotton Conferences pg. 610
©National Cotton Council, Memphis TN

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Document last modified Sunday, Dec 6 1998