Tannin Stimulates Arachidonic Acid Release from Bovine Tracheal Epithelial Cells

Michelle M. Cloutier and Linda Guernsey


 
ABSTRACT

Tannin increases arachidonic acid (AA) release in rabbit alveolar macrophages and inhibits its subsequent reacylation. The purpose of this study was to determine whether tannin had any effect upon arachidonic acid release in bovine tracheal epithelial cells (BTE). (14)C-AA release was measured at timed intervals after addition of various concentrations of tannin in BTE cells grown to confluence in the presence of (14)C-AA. Tannin caused a time and dose-dependent release of AA from airway cells with a maximum release occurring at 1 minute in the presence of 100 µ/ml of tannin. AA release by tannin was not inhibited by indomethacin, a cyclooxygenase pathway inhibitor or ETYA, an inhibitor of the lipoxygenase pathway of prostaglandin metabolism. Both of these drugs were however, effective in inhibiting bradykinin induced AA release. In addition, AA release was not inhibited by cycloheximide, an inhibitor of protein synthesis. These data suggest that tannin increases AA release by bovine tracheal epithelial cells and that the AA released is not metabolized by either the cyclooxygenase or the lipoxygenase pathway.



Reprinted from 1993 Proceedings Beltwide Cotton Conferences pp. 340 - 342
©National Cotton Council, Memphis TN

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Document last modified Sunday, Dec 6 1998