Selective Media for Separating Gliovirin and Gliotoxin Producing Strains of Gliocladium Virens

Charles R. Howell


 
ABSTRACT

Strains of Gliocladium virens can be divided into groups on the basis of their growth and sporulation on differential agar media. On PDA containing 0.5 µg/ml benlate, P strains (gliovirin producers) grow well, whereas the growth of Q strains (gliotoxin producers) is restricted. On PDA containing 50 µg/ml of bliotoxin, Q strains grow well and sporulate profusely, whereas the growth of P strains is reduced and sporulation is severely restricted. Strains within the P group can be further segregated on PDA containing 1 µg/ml flusilazol, which restricts the growth of some strains more than others. Assays of 22 strains of G. virens isolated from 8 cotton growing states showed an absolute correlation between antibiotic production and growth response to inhibitors, indicating that it is feasible to screen soil for strains from a particular group by adding the appropriate inhibitor to the growth medium. Growth responses to inhibitors may also be used as phenotypic markers to detect recombinant strains resulting from crosses between P and Q strains that will grow on media containing both benlate and gliotoxin.



Reprinted from 1993 Proceedings Beltwide Cotton Conferences pg. 223
©National Cotton Council, Memphis TN

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Document last modified Sunday, Dec 6 1998