Analysis of Histamine in Biological Fluids by High- Performance Liquid Chromatography

J.H. Wall, R.J. Berni, and M. G. Buck


Two HPLC methods for histamine determination are recommended. Urinary histamine can best be determined using a concentration/cleanup technique with postcolumn derivatization with o-phthaldehyde (OPA). Plasma histamine, because of low natural occurrence and limited sample, is best determined by a precolumn derivatization with OPA. A brief description of technique and chromatograms are presented. Lower limits of detection for both methods are in the picogram range.

Reprinted from Proceedings of the 1983 Beltwide Cotton Dust Conference pp. 114 - 118
©National Cotton Council, Memphis TN

[Main TOC] | [TOC] | [TOC by Section] | [Search] | [Help]
Previous Page [Previous] [Next] Next Page
Document last modified Sunday, Dec 6 1998