Excellent correlations exist between the three currently available methods for measuring short fiber content in cotton, i.e., the Suter-Webb Array, Peyer AL101, and Zellweger Uster AFIS L & D. Each unit, however, is on a slightly different level from the other two, mainly as a result of sample preparation. In the experiments comparing instruments, a study was also performed to determine if HVI Uniformity Index was a good measure of short fiber content. The results showed poor correlation between the two. Taking two levels of short fiber content into a spinning mill's mix laydown showed that short fiber content adversely affected process performance and yarn quality.