'Chembred 1135', 'Coker 315', 'Coker 320', 'Deltapine 50', 'Deltapine 90', and 'PD 3' cotton cultivars were evaluated for their response to infection by Columbia lance (Hoplolaimus columbus), reniform (Rotylenchulus reniformis), and root-knot (Meloidogyne incognita) nematodes. Plots were established in three fields. The first field (a Lucy loamy sand) had a Columbia lance nematode infestation level of 82/100 cm³ of soil, the second field (an Orangeburg loam sand) a root-knot nematode level of 6/100 cm³ soil, and the third field (a Marlboro sandy loam) 51 Columbia lance + 635 reniform/100 cm³ soil. Each cultivar was replicated 6 times in a strip-block design with paired nontreated and nematicide treated plots of each cultivar. Nematicide treatment consisted of 7 lb/A of Temik 15G applied in-furrow at planting. Plots consisted of 4 50-ft long rows on 38 inch centers. All plots were sprayed 5, 10, and 15 days after planting for thrips control. Percent yield loss was calculated as ((treated yield-nontreated yield)/(treated yield)) x 100. Mean yield losses across all cultivars were: Columbia lance field-15%, root-knot field-11%, and reniform + root-knot field-10%. Nematicide treatment reduced recovery of Columbia lance and reniform nematodes and galling by root-knot nematode and subsequently increased mean yields across all cultivars. Cultivars did not exhibit varying levels of resistance to infection or yield loss for Columbia lance or root-knot nematodes, however in the reniform field nontreated PD 3 appeared to sustain higher yield losses than all other nontreated cultivars except Deltapine 90 and also exhibited the highest percent yield loss.