The use of high temperatures to destroy endotoxins is a well-established technique for medical devices, laboratory glassware, etc. In the case of cotton fibers, excessively high temperatures can produce cellulose degradation and damage to fiber surface or mechanical properties, resulting in substandard performance in processing. With heated metal plates or rollers, a combination of short-time and high-temperature might result in reduced endotoxin content without detrimental effects on the fibers. In preliminary experiments, cotton sliver and bulk weathered cotton fiber were heated for times ranging from 0.5 to 6.0 minutes between metal plates heated to temperatures near 200 degrees C. The effects of the heat treatments on endotoxin content, fiber strength, color, and dye uptake were determined.