Field studies were conducted in 1987-1989 to evaluate cotton production in both 30- and 40-inch rows. Seed cotton yield on Bosket very fine sandy loam soil was not significantly influenced by row spacing. Seed cotton yield on Dundee silty clay loam was significantly higher for 30-inch rows in 1988 and 1989 when compared to 40-inch rows. The three year average seed cotton yield for the 30-inch rows compared to the 40-inch rows was increased by 7% for the Bosket soil and 19% for the Dundee silty clay loam. The three year average seed cotton yield per land acre from three 30-inch rows in a wide bed, controlled traffic production system was 10% higher than yield from two 40-inch rows. Yield from plots treated with the plant growth regulator Pix out yielded the nontreated plots but the difference was not significant. The 30-inch row concept appears to be more advantageous on the mixed and clay soils of the Delta or in specialized systems such as controlled traffic.