A better understanding of cotton fiber cell elongation may be obtained by assessing the effects of plant growth regulators on calls derived from cotton ovules. Cotton callus tissue was initiated from 7 days preanthesis ovules. The callus was grown on a modified Murashige and Skoog medium containing 2,4-D (.1 mg/1), kinatin (.5 mg/1), glucose (30 g/1) and agar (8.75 g/1). After several passages in culture cells were transferred to liquid Beasley and Ting medium. Gibberellic acid, 2,4-D, naphthalene acetic acid, isopentenyl adenine and kinetin were added in various combinations. The addition of gibberellic acid alone or in combination with other growth regulators promoted elongation. The similarities between cell elongation in cotton fibers and cotton cell cultures will be discussed.