As part of an effort to develop procedures for more rapid production of transgenic cotton plants, the possibility of regenerating plants from roots was explored. Root explants of two cotton cultivars were excised from seedlings and placed onto 16 different media. Calli were subsequently obtained and proliferated by plating onto callus proliferation medium. Embryogenic callus arising after 3 months of culture was maintained on embryo maintenance medium for 9 months. Mature somatic embryos transferred to embryo germination medium gave rise to shootlets which were rooted. Plants were then transferred to soil and hardened in a greenhouse.