A glasshouse study was conducted over a two-year period to determine the effects of nitrogen on cotton (Gossypium hirsutum L.) fruiting, boll development, and fiber properties. Leaf nitrogen ranged from one to three percent during fruiting and boll maturation for the lowest and highest nitrogen treatment, respectively. Nitrogen stress reduced the plant leaf area, boll number, and weight of individual bolls. Boll number per plant was reduced to a greater extent by nitrogen stress than boll weight. The boll maturation period was unaffected by nitrogen. Nitrogen stress, mainstem boll location, and sympodial branch position affected fiber weight/boll. However, fiber uniformity was the only fiber quality property affected by plant nitrogen stress. Bolls at the second sympodial positions had lower values for micronaire, fiber length, fiber strength, and uniformity, but greater elongation than bolls at the first sympodial position.