Pulmonary Epithelium as a Source of Neutrophil Chemotactic Factors (NCF): Tannin-Mediated Ncf Secretion from A549 Cells.

Andre DeLorme, Teresa Kreofsky and Michael S. Rohrbach


 
ABSTRACT

Previous studies demonstrated that inhalation of cotton dust extract or condensed tannin isolated from cotton dust in a rabbit model resulted in the development of an acute pulmonary inflammatory response characterized by the presence of increased levels of neutrophils and NCF in the airways. The source of the NCF in these studies was unknown. One potential cellular source of the NCF was the epithelial cells lining the airways and alveoli. To test this possibility, A549 cells, a continuous transformed cell line derived from human alveolar type 11 cells, were exposed to various concentrations of tannin for periods ranging from 1 minute to 120 minutes and the supernatant assayed for NCF activity. These studies demonstrated that tannin promoted the dose-dependent release of NCF with maximal secretion occurring at a tannin concentration of 50 µg/ml. The release was extremely rapid, ie completed in 1 minute, when compared to the tannin-dependent release of NCF from rabbit alveolar macrophages. These results suggest that the airway epithelial cells may be a potent source of rapidly released NCF following inhalation of cotton dust or tannin.



Reprinted from Proceedings: 1989 Beltwide Cotton Dust Conference pp. 82 - 84
©National Cotton Council, Memphis TN

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Document last modified Sunday, Dec 6 1998