Acute Respiratory Response of Guinea Pigs to Lipopolysaccharide from Salmonella minnesota and the RE595 Mutant

Lisa K. Ryan, Serap Erdal, and Meryl H. Karol


 
ABSTRACT

Purified endotoxin from Enterobacter agglomerans, Pseudomonas syringae and Escherichia coli have been found to produce rapid shallow breathing in guinea pigs. This respiratory response was similar to that seen with guinea pigs exposed to cotton dust. Using the guinea pig animal model for byssinosis, the current study investigated the respiratory effects of Salmonella Minnesota lipopolysaccharide (LPS) and compared it with the respiratory activity of LPS from E. agglomerans, Ps. syringae, and E. coli 0111:B4. In addition, the respiratory activity of S. minnesota wild type LPS was compared with that of its Re mutant. Guinea pigs were exposed for 6 hr to aerosols of purified LPS. LPS from S. Minnesota had similar respiratory toxicity to the other endotoxine and the respiratory potency of the LPS from the wild type and Re mutant bacteria were indistinguishable. These findings indicate that the polysaccharide component of LPS may have little importance in determining the respiratory toxicity of endotoxin.



Reprinted from Cotton Dust: Proceedings-12th Cotton Dust Research Conference 1988 pp. 47 - 49
©National Cotton Council, Memphis TN

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Document last modified Sunday, Dec 6 1998