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Chaperoneā„¢ Results from the Tri-State Delta

J.H. Lackey, D.M. Oosterhuis, and R.S. Brown

ABSTRACT

Variability of endotoxin protein expression and/or concentration within transgenic cotton (Bt) varieties has been and continues to be a concern of cotton growers, researchers and many breeders. While not a consistent problem, it is one which can cause major economic problems. Transgenic cotton varieties, to have effective insecticidal properties, must produce additional levels of proteins that conventional cottons do not. Cotton needs high levels of protein to maintain high yields, whereas transgenic cottons require additional protein to sustain insecticidal endotoxin proteins. Chaperone, a protein transport enhancer, containing nitrophenolates has been shown to increase the efficacy of endotoxin protein levels within transgenic cottons, resulting in higher worm mortality and yield. Chaperone has increased endotoxin protein levels in leaves and squares, and feeding trials have shown an increase in the percentage of worm mortality. In addition, Chaperone, through its protein binding and translocation capabilities, has produced yield increases even at low worm threshold levels.





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Document last modified 04/27/04