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High-Throughput Development of New Molecular Markers for Cotton

Michael B. Palmer, Dorrie Main, James Frelichowski, Jeffrey P. Tomkins, and Mauricio Ulloa

ABSTRACT

The ubiquity of simple sequence repeats (SSRs) in eukaryotic genomes and their usefulness as genetic markers is now well established. A recent advance in molecular marker development has been the mining of simple sequence repeat (SSR) markers from BAC-end sequence data. SSRs are desirable for use in marker systems because they are highly polymorphic, abundant, widely dispersed throughout eukaryotic genomes, transportable between laboratories, and are inherited in a co-dominant fashion. This approach provides a rich and novel source of new PCR-based markers, and the markers that are identified can be traced back to specific large-insert clones in the public cotton BAC library. This advantage has further applications towards map-based cloning and physical mapping of the entire cotton genome. Here we report on the development of SSRs from random sequencing of BAC clones from a large-insert library derived from the elite Upland cultivar Gossypium hirsutum L. Maxxa.





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Document last modified 04/27/04