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Molecular Characterisation and Assessment of the Resistance to the Renifom Nematode of an Interspecific Hybrid Involving Gossypium hirsutum and G. longicalyx

N.O. Konan, J-P Baudoin, and G. Mergeai

ABSTRACT

Two hundred and three mapped micro-satellites markers evenly distributed on the 26 chromosomes of G. hirsutum L. were used to monitor the introgression of DNA fragments coming from the Australian species G. sturtianum Willis in a population of BC1, BC2, BC2S1, BC2S2, BC2S3, B22S4, BC2S5, BC1/BC2S2, S1/BC1/BC2S2, BC3, BC3S1, BC3S2 and BC3S3 backcross derivatives obtained from the G. hirsutum xG.raimondiiUlb. x G. sturtianum (HRS) trispecific hybrid. A single marker analysis carried out in this population permitted to associate six G. sturtianum microsatellites to putative QTLs controlling the low gossypol-seed and high-gossypol plant trait. These microsatellites are located on chromosomes c02, c13 and c12. They constitute very useful tools to assist the selection of commercial cotton varieties showing a drastic inhibition of the gossypol synthesis in the seed while presenting a normal content of terpenoids in their aerial parts.





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Document last modified 04/27/04