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Profiling Seasonal Expression of Cry2Ab in Bolls of Dual-Toxin Bt Cotton

D.S. Akin, M.B. Layton, S.D. Stewart, and John J. Adamczyk, Jr.

ABSTRACT

Laboratory studies were conducted to quantify toxin levels in bolls located at various locations throughout dual-toxin Bt cotton plants. Results of ELISA techniques indicate that there was a significant quadratic relationship between node location and Cry2Ab concentration, with expression levels highest in bolls from the central portion of the plant. For bolls at node 9 and above (averaged over positions 1 and 2), there was a significant decline in Cry2 expression as the season progressed. A more dramatic reduction of Cry2Ab levels was noted above node 16 in bolls from position 1and node 14 in bolls from position 2. Since bolls from these locations are from the same cohort (i.e., the same phenological age), this would indicate that environmental factors may play a significant role in expression of Bt toxins throughout the plant.





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Document last modified April 16, 2003