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Somatic Embryogenesis Ability in Select Georgia and Pee Dee Cotton Lines

Hamidou F. Sakhanokho, Peggy Ozias-Akins, O. Lloyd May, and Peng W. Chee

ABSTRACT

Fifteen Georgia and Pee Dee cotton germplasm lines along with Coker 312 (used as a positive control) were screened for their regenerability potential using nine embryo initiation/development media. In addition to Coker 312, somatic embryos and plants were obtained in PD 97019, PD 97021 and PD 98033 lines. Close to 95% of the Coker 312 seeds tested produced somatic embryos. However, extensive seed to seed variation was found in the regenerable Pee Dee lines. Efforts are under way to evaluate the effects of the various media used and develop pure regenerable lines to improve the regeneration efficiency of these newly found responsive Pee Dee genotypes.





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Document last modified April 16, 2003