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Presence of Nitric Oxide Synthase Activity Under NaCl-Induced Stress in Cotton Callus

A.M. Virgen, D.R. Gossett, S.W. Banks and M.C. Lucas


ABSTRACT

The presence of nitric oxide synthase (NOS) in cotton callus Coker 312 was studied. The immunoblot analysis of cytosolic extracts with a polyclonal antibody against the C-terminus region of murine iNOS revealed an immunoreactive protein of 130kDa. The evidence presented by measuring the conversion of L-[14C]arginine to L-[14C]citrulline assay indicates the production of nitric oxide as an adaptive mechanism of plants under NaCl induced physiological stress.





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Document last modified May 20, 2002